GUÁMAN BAUTISTA, LINDA PRISCILA

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Facultad de Ciencias de la Salud Eugenio Espejo

CENBIO - Centro de Investigación Biomédica

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57190735993

Now showing 1 - 10 of 28

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Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures
(S. Karger AG, 2018)
Edmar Ramos Oliveira-Filho
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GUAMAN BAUTISTA, LINDA PRISCILA
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Thatiane Teixeira Mendonça
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Paul F. Long
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Marilda Keico Taciro
<jats:p>Three different polyhydroxyalkanoate (PHA) synthase genes (Ralstonia eutropha H16, Aeromonas sp. TSM81 or Aeromonas hydrophila ATCC7966 phaC) were introduced into the chromosome of two Pseudomonas strains: a native medium-chain-length 3-polyhydroxyalkanoate (PHAMCL) producer (Pseudomonas sp. LFM046) and a UV-induced mutant strain unable to produce PHA (Pseudomonas sp. LFM461). We reported for the first time the insertion of a chromosomal copy of phaC using the transposon system mini-Tn7. Stable antibiotic marker-free and plasmid-free recombinants were obtained. Subsequently, P(3HB-co-3HAMCL) was produced by these recombinants using glucose as the sole carbon source, without the need for co-substrates and under antibiotic-free conditions. A recombinant harboring A. hydrophila phaC produced a terpolyester composed of 84.2 mol% of 3-hydroxybutyrate, 6.3 mol% of 3-hydroxyhexanoate, and 9.5 mol% of 3-hydroxydecanoate from only glucose. Hence, we were successful in increasing the industrial potential of Pseudomonas sp. LFM461 strain by producing PHA copolymers containing 3HB and 3HAMCL using an unrelated carbon source, for the first time in a plasmid- and antibiotic-free bioprocess.</jats:p>
Scopus© Citations 6
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Crystal Structure, Hirshfeld Surface Analysis, and Biological Activities of Schiff-Base Derivatives of 4-Aminoantipyrine
(American Chemical Society (ACS), 2023-10-31)
Esteban Aguilar-Llanos
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Saskya E. Carrera-Pacheco
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GONZALEZ PASTOR, REBECA EUGENIA
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Juan Carlos Romero-Benavides
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Carlos Cevallos-Morillo
Scopus© Citations 32
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Green Synthesis of Silver Oxide Nanoparticles from Mauritia flexuosa Fruit Extract: Characterization and Bioactivity Assessment
(MDPI AG, 2024-11-22)
ZUÑIGA MIRANDA, JOHANA JANINA
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David Vaca-Vega
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Karla Vizuete
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Saskya E. Carrera-Pacheco
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GONZALEZ PASTOR, REBECA EUGENIA
The increasing prevalence of multidrug-resistant (MDR) pathogens, persistent biofilms, oxidative stress, and cancerous cell proliferation poses significant challenges in healthcare and environmental settings, highlighting the urgent need for innovative and sustainable therapeutic solutions. The exploration of nanotechnology, particularly the use of green-synthesized nanoparticles, offers a promising avenue to address these complex biological challenges due to their multifunctional properties and biocompatibility. Utilizing a green synthesis approach, Mauritia flexuosa Mf-Ag2ONPs were synthesized and characterized using dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy coupled with scanning electron microscopy (EDS-SEM), UV-Vis spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The Mf-Ag2ONPs exhibited potent antibacterial effects against both non-resistant and MDR bacterial strains, with minimum inhibitory concentrations (MICs) ranging from 11.25 to 45 µg/mL. Mf-Ag2ONPs also demonstrated significant antifungal efficacy, particularly against Candida glabrata, with an MIC of 5.63 µg/mL. Moreover, the nanoparticles showed strong biofilm inhibition capabilities and substantial antioxidant properties, underscoring their potential to combat oxidative stress. Additionally, Mf-Ag2ONPs exhibited pronounced anticancer properties against various cancer cell lines, displaying low IC50 values across various cancer cell lines while maintaining minimal hemolytic activity at therapeutic concentrations. These findings suggest that Mf-Ag2ONPs synthesized via an eco-friendly approach offer a promising alternative for biomedical applications, including antimicrobial, antifungal, antioxidant, and anticancer therapies, warranting further in vivo studies to fully exploit their therapeutic potential.
Scopus© Citations 15
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Evaluation of Biological Activity of Natural Compounds: Current Trends and Methods
(MDPI AG, 2022-07-13)
Carlos Barba-Ostria
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Saskya E. Carrera-Pacheco
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GONZALEZ PASTOR, REBECA EUGENIA
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Cristina Rodríguez-Pólit
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HEREDIA MOYA, JORGE HUMBERTO
<jats:p>Natural compounds have diverse structures and are present in different forms of life. Metabolites such as tannins, anthocyanins, and alkaloids, among others, serve as a defense mechanism in live organisms and are undoubtedly compounds of interest for the food, cosmetic, and pharmaceutical industries. Plants, bacteria, and insects represent sources of biomolecules with diverse activities, which are in many cases poorly studied. To use these molecules for different applications, it is essential to know their structure, concentrations, and biological activity potential. In vitro techniques that evaluate the biological activity of the molecules of interest have been developed since the 1950s. Currently, different methodologies have emerged to overcome some of the limitations of these traditional techniques, mainly via reductions in time and costs. These emerging technologies continue to appear due to the urgent need to expand the analysis capacity of a growing number of reported biomolecules. This review presents an updated summary of the conventional and relevant methods to evaluate the natural compounds’ biological activity in vitro.</jats:p>
Scopus© Citations 150
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Mxenes as a versatile nanoplatform: Synthesis and emerging biomedical applications
(Elsevier BV, 2025-09-25)
Ali Mohammad Amani
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Ehsan Vafa
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Maryam Mirzae
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Milad Abbasi
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Ahmad Vaez
Recently, MXenes have garnered significant attention in academic research due to their remarkable structural, electrical, magnetic, optical, mechanical, and chemical properties. New advancements and emerging findings indicate that MXene, classified as a two-dimensional (2D) layered material, exhibits significantly more potential in the field of biomedicine and biotechnology compared to existing nanosystems. These applications include acting as antibacterial agents, biosensor systems, the delivery and loading of drugs, bioimaging, and therapeutic interventions. The unique characteristics of MXenes, such as their significant electrical conductivity, large surface area, low toxicity, magnetism, luminescence, and high biocompatibility, make them highly promising candidates for diagnostic, therapeutic, and theranostic applications. Surface modifications of MXenes exhibit biocompatibility and have multifunctional functions, including the ability to direct ligands towards specific spots for preferential aggregation, hence enabling their utilization in specialized applications. This paper provides an overview of the characteristics, modifications, and synthesis methods of MXene nanomaterials. The present article also delivers a comprehensive assessment of the practical uses of MXene-based nanomaterials in biomedicine, with a particular focus on biosensing, bioimaging, antibacterial effects, implants, and multifunctional therapeutic platforms. This paper also presents a discussion of the future prospects and challenges associated with the applications of MXenes in the biomedicine field.
Scopus© Citations 38
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Iron Oxide Nanoparticles: Green Synthesis and Their Antimicrobial Activity
(MDPI AG, 2023-11-08)
ZUÑIGA MIRANDA, JOHANA JANINA
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Julio Guerra
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Alexander Mueller
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MAYORGA RAMOS, ARIANNA CAROLINA
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Carlos Barba-Ostria
<jats:p>The rise of antimicrobial resistance caused by inappropriate use of these agents in various settings has become a global health threat. Nanotechnology offers the potential for the synthesis of nanoparticles (NPs) with antimicrobial activity, such as iron oxide nanoparticles (IONPs). The use of IONPs is a promising way to overcome antimicrobial resistance or pathogenicity because of their ability to interact with several biological molecules and to inhibit microbial growth. In this review, we outline the pivotal findings over the past decade concerning methods for the green synthesis of IONPs using bacteria, fungi, plants, and organic waste. Subsequently, we delve into the primary challenges encountered in green synthesis utilizing diverse organisms and organic materials. Furthermore, we compile the most common methods employed for the characterization of these IONPs. To conclude, we highlight the applications of these IONPs as promising antibacterial, antifungal, antiparasitic, and antiviral agents.</jats:p>
Scopus© Citations 153
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High—throughput and automated screening for COVID-19
(Frontiers Media SA, 2022-09-15)
Nestor Jonguitud-Borrego
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Koray Malcı
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Mihir Anand
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Erikan Baluku
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Calum Webb
<jats:p>The COVID-19 pandemic has become a global challenge for the healthcare systems of many countries with 6 million people having lost their lives and 530 million more having tested positive for the virus. Robust testing and a comprehensive track and trace process for positive patients are essential for effective pandemic control, leading to high demand for diagnostic testing. In order to comply with demand and increase testing capacity worldwide, automated workflows have come into prominence as they enable high-throughput screening, faster processing, exclusion of human error, repeatability, reproducibility and diagnostic precision. The gold standard for COVID-19 testing so far has been RT-qPCR, however, different SARS-CoV-2 testing methods have been developed to be combined with high throughput testing to improve diagnosis. Case studies in China, Spain and the United Kingdom have been reviewed and automation has been proven to be promising for mass testing. Free and Open Source scientific and medical Hardware (FOSH) plays a vital role in this matter but there are some challenges to be overcome before automation can be fully implemented. This review discusses the importance of automated high-throughput testing, the different equipment available, the bottlenecks of its implementation and key selected case studies that due to their high effectiveness are already in use in hospitals and research centres.</jats:p>
Scopus© Citations 16
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Exploring the Multifaceted Biological Activities of Anthocyanins Isolated from Two Andean Berries
(MDPI AG, 2024-08-21)
Carlos Barba-Ostria
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Saskya E. Carrera-Pacheco
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GONZALEZ PASTOR, REBECA EUGENIA
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ZUÑIGA MIRANDA, JOHANA JANINA
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MAYORGA RAMOS, ARIANNA CAROLINA
Natural pigments extracted from plant species are used in foods, cosmetics, and pharmaceuticals. This study evaluates the comprehensive biological activities of anthocyanins isolated from Andean blueberry (Vaccinium floribundum Kunth) and Andean blackberry (Rubus glaucus Benth), focusing on their antimicrobial, antioxidant, antitumoral, anti-inflammatory, and hemolytic properties. Chemical characterization revealed significant anthocyanin content with complex mass spectrometric profiles indicating diverse glycosylation patterns that may influence their bioactivity. The antimicrobial assays showed that the extracts were particularly effective against Gram-positive bacteria, with minimal inhibitory concentrations (MICs) as low as 1 mg/mL for Rubus glaucus, indicating strong potential for therapeutic use. The antioxidant capacity of the berries was substantial, albeit slightly lower than that of ascorbic acid. The extracts also exhibited notable antitumoral activity in various cancer cell lines, showing promise as adjunctive or preventive treatments. The anti-inflammatory effects were confirmed by inhibiting nitric oxide production in macrophage cells, highlighting their potential in managing inflammatory diseases. In terms of hemolytic activity, Rubus glaucus exhibited dose-dependent effects, potentially attributable to anthocyanins and phenolics, while Vaccinium floribundum demonstrated no significant hemolytic activity, underscoring its safety. These findings suggest that anthocyanins from Andean berries possess potent biological activities, which could be leveraged for health benefits in pharmaceutical and nutraceutical applications. Further studies are needed to isolate specific bioactive compounds and investigate their synergistic effects in clinical and real-world contexts.
Scopus© Citations 13
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Experimental and computational studies of Schiff bases derived from 4-aminoantipyrine as potential antibacterial and anticancer agents
(Springer Science and Business Media LLC, 2025-01-31)
ZUÑIGA MIRANDA, JOHANA JANINA
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GONZALEZ PASTOR, REBECA EUGENIA
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Saskya E. Carrera Pacheco
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Cristina Rodríguez-Pólit
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Carlos Barba-Ostria
Schiff bases are organic compounds recognized for their biological activities, including antiviral, antibacterial, antifungal, and anticancer properties, making them promising candidates in medicinal chemistry. In this studio, a series of Schiff bases derived from 4-aminoantipyrine and substituted cinnamaldehydes were evaluated in vitro against liver (HepG2) and thyroid (THJ29T) cancer cells, Gram-positive and Gram-negative multidrug-resistant bacteria, and biofilm-forming pathogens. Six compounds demonstrated anticancer activity, though some exhibited toxicity to non-tumor cells. Compounds showed notable anticancer potential, while also exhibited strong antibacterial effects, with being the most effective against multidrug-resistant bacteria strains. These Schiff bases also inhibit biofilm formation, suggesting their potential for treating biofilm-related infections. analyses of their ADME properties, global reactivity descriptors, and binding affinities corroborated these findings. The Schiff base has a strong binding affinity for DNA gyrase and vitamin D receptor, suggesting potential mechanisms for its antibacterial and anticancer activities.
Scopus© Citations 20
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Advances in environmental biotechnology with CRISPR/Cas9: bibliometric review and cutting-edge applications
(Springer Science and Business Media LLC, 2025-02-22)
Jessica Verdezoto Prado
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Cristhian Chicaiza Ortiz
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Ana Belén Mejía Pérez
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Camila Freire Torres
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Marco Viteri Yánez
CRISPR/Cas9 has emerged as the predominant method for genome editing due to its cost-effectiveness and broad applicability, playing a crucial role in advancing sustainable practices across various sectors. This systematic review employs the PRISMA methodology to evaluate the impact of CRISPR/Cas9 on environmental protection and on achieving Sustainable Development Goals (SDGs) such as SDG 2, 3, 6, 7, 9, 12, 13, and SDG15. These goals focus on the responsible use of natural resources, reducing the negative effects of climate change and ensuring safe food for the entire population. Analyzing data from the Web of Science, the review found significant growth in related publications, with a 30% increase since 2014, predominantly from the US, China, Germany, and the UK. The study categorizes the scientific developments into these trends, the enhancement of plant tolerance to environmental stresses, as evidenced by the consistent focus on terms such as “tolerance” and “plant” since 2021. Furthermore, the relevance of “Gene Editing” has increased significantly since 2022, underscoring the importance of CRISPR/Cas9 in developing resilient crops that can withstand extreme conditions. These trends underscore the growing significance of biotechnological advancements in the mitigation of climate change’s effects and the improvement of ecosystem stability. Key discussions include CRISPR/Cas9’s role in the development of fourth-generation biofuels and environmental biosensors, as well as its applications in enhancing genetic resilience and controlling invasive species. These innovations highlight CRISPR/Cas9’s potential in promoting sustainable resource management and energy generation, making a significant contribution to ecological conservation and sustainability efforts.
Scopus© Citations 32

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