GUÁMAN BAUTISTA, LINDA PRISCILA

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GUÁMAN BAUTISTA, LINDA PRISCILA
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CENBIO - Centro de Investigación Biomédica
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57190735993

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    Green Synthesis of Silver Oxide Nanoparticles from Mauritia flexuosa Fruit Extract: Characterization and Bioactivity Assessment
    (MDPI AG, 2024-11-22)
    ZUÑIGA MIRANDA, JOHANA JANINA  
    ;
    David Vaca-Vega
    ;
    Karla Vizuete
    ;
    Saskya E. Carrera-Pacheco
    ;
    GONZALEZ PASTOR, REBECA EUGENIA  
    The increasing prevalence of multidrug-resistant (MDR) pathogens, persistent biofilms, oxidative stress, and cancerous cell proliferation poses significant challenges in healthcare and environmental settings, highlighting the urgent need for innovative and sustainable therapeutic solutions. The exploration of nanotechnology, particularly the use of green-synthesized nanoparticles, offers a promising avenue to address these complex biological challenges due to their multifunctional properties and biocompatibility. Utilizing a green synthesis approach, Mauritia flexuosa Mf-Ag2ONPs were synthesized and characterized using dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy coupled with scanning electron microscopy (EDS-SEM), UV-Vis spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The Mf-Ag2ONPs exhibited potent antibacterial effects against both non-resistant and MDR bacterial strains, with minimum inhibitory concentrations (MICs) ranging from 11.25 to 45 µg/mL. Mf-Ag2ONPs also demonstrated significant antifungal efficacy, particularly against Candida glabrata, with an MIC of 5.63 µg/mL. Moreover, the nanoparticles showed strong biofilm inhibition capabilities and substantial antioxidant properties, underscoring their potential to combat oxidative stress. Additionally, Mf-Ag2ONPs exhibited pronounced anticancer properties against various cancer cell lines, displaying low IC50 values across various cancer cell lines while maintaining minimal hemolytic activity at therapeutic concentrations. These findings suggest that Mf-Ag2ONPs synthesized via an eco-friendly approach offer a promising alternative for biomedical applications, including antimicrobial, antifungal, antioxidant, and anticancer therapies, warranting further in vivo studies to fully exploit their therapeutic potential.
    Scopus© Citations 14
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    Mxenes as a versatile nanoplatform: Synthesis and emerging biomedical applications
    (Elsevier BV, 2025-09-25)
    Ali Mohammad Amani
    ;
    Ehsan Vafa
    ;
    Maryam Mirzae
    ;
    Milad Abbasi
    ;
    Ahmad Vaez
    Recently, MXenes have garnered significant attention in academic research due to their remarkable structural, electrical, magnetic, optical, mechanical, and chemical properties. New advancements and emerging findings indicate that MXene, classified as a two-dimensional (2D) layered material, exhibits significantly more potential in the field of biomedicine and biotechnology compared to existing nanosystems. These applications include acting as antibacterial agents, biosensor systems, the delivery and loading of drugs, bioimaging, and therapeutic interventions. The unique characteristics of MXenes, such as their significant electrical conductivity, large surface area, low toxicity, magnetism, luminescence, and high biocompatibility, make them highly promising candidates for diagnostic, therapeutic, and theranostic applications. Surface modifications of MXenes exhibit biocompatibility and have multifunctional functions, including the ability to direct ligands towards specific spots for preferential aggregation, hence enabling their utilization in specialized applications. This paper provides an overview of the characteristics, modifications, and synthesis methods of MXene nanomaterials. The present article also delivers a comprehensive assessment of the practical uses of MXene-based nanomaterials in biomedicine, with a particular focus on biosensing, bioimaging, antibacterial effects, implants, and multifunctional therapeutic platforms. This paper also presents a discussion of the future prospects and challenges associated with the applications of MXenes in the biomedicine field.
    Scopus© Citations 37
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    Scopus© Citations 182
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    Current Landscape of Methods to Evaluate Antimicrobial Activity of Natural Extracts
    (MDPI AG, 2023-01-20)
    GONZALEZ PASTOR, REBECA EUGENIA  
    ;
    Saskya E. Carrera-Pacheco
    ;
    ZUÑIGA MIRANDA, JOHANA JANINA  
    ;
    Cristina Rodríguez-Pólit
    ;
    MAYORGA RAMOS, ARIANNA CAROLINA  
    <jats:p>Natural extracts have been and continue to be used to treat a wide range of medical conditions, from infectious diseases to cancer, based on their convenience and therapeutic potential. Natural products derived from microbes, plants, and animals offer a broad variety of molecules and chemical compounds. Natural products are not only one of the most important sources for innovative drug development for animal and human health, but they are also an inspiration for synthetic biology and chemistry scientists towards the discovery of new bioactive compounds and pharmaceuticals. This is particularly relevant in the current context, where antimicrobial resistance has risen as a global health problem. Thus, efforts are being directed toward studying natural compounds’ chemical composition and bioactive potential to generate drugs with better efficacy and lower toxicity than existing molecules. Currently, a wide range of methodologies are used to analyze the in vitro activity of natural extracts to determine their suitability as antimicrobial agents. Despite traditional technologies being the most employed, technological advances have contributed to the implementation of methods able to circumvent issues related to analysis capacity, time, sensitivity, and reproducibility. This review produces an updated analysis of the conventional and current methods to evaluate the antimicrobial activity of natural compounds.</jats:p>
    Scopus© Citations 90
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    Protective role of butyrate in obesity and diabetes: New insights
    (Frontiers Media SA, 2022-11-24)
    MAYORGA RAMOS, ARIANNA CAROLINA  
    ;
    Carlos Barba-Ostria
    ;
    SIMANCAS RACINES, DANIEL ALEJANDRO
    ;
    GUAMAN BAUTISTA, LINDA PRISCILA  
    <jats:p>Studies in human microbiota dysbiosis have shown that short-chain fatty acids (SCFAs) like propionate, acetate, and particularly butyrate, positively affect energy homeostasis, behavior, and inflammation. This positive effect can be demonstrated in the reduction of butyrate-producing bacteria observed in the gut microbiota of individuals with type 2 diabetes (T2DM) and other energy-associated metabolic alterations. Butyrate is the major end product of dietary fiber bacterial fermentation in the large intestine and serves as the primary energy source for colonocytes. In addition, it plays a key role in reducing glycemia and improving body weight control and insulin sensitivity. The major mechanisms involved in butyrate regulation include key signaling pathways such as AMPK, p38, HDAC inhibition, and cAMP production/signaling. Treatment strategies using butyrate aim to increase its intestine levels, bioavailability, and improvement in delivery either through direct supplementation or by increasing dietary fiber in the diet, which ultimately generates a higher production of butyrate in the gut. In the final part of this review, we present a summary of the most relevant studies currently being carried out in humans.</jats:p>
    Scopus© Citations 111
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    The Impact of Bioactive Molecules from Probiotics on Child Health: A Comprehensive Review
    (MDPI AG, 2024-10-30)
    GUAMAN BAUTISTA, LINDA PRISCILA  
    ;
    Saskya E. Carrera-Pacheco
    ;
    ZUÑIGA MIRANDA, JOHANA JANINA  
    ;
    Enrique Teran
    ;
    Cesar Erazo
    <jats:p>Background: This review investigates the impact of bioactive molecules produced by probiotics on child health, focusing on their roles in modulating gut microbiota, enhancing immune function, and supporting overall development. Key metabolites, including short-chain fatty acids (SCFAs), bacteriocins, exopolysaccharides (EPSs), vitamins, and gamma-aminobutyric acid (GABA), are highlighted for their ability to maintain gut health, regulate inflammation, and support neurodevelopment. Objectives: The aim of this review is to examine the mechanisms of action and clinical evidence supporting the use of probiotics and postbiotics in pediatric healthcare, with a focus on promoting optimal growth, development, and overall health in children. Methods: The review synthesizes findings from clinical studies that investigate the effects of probiotics and their metabolites on pediatric health. The focus is on specific probiotics and their ability to influence gut health, immune responses, and developmental outcomes. Results: Clinical studies demonstrate that specific probiotics and their metabolites can reduce gastrointestinal disorders, enhance immune responses, and decrease the incidence of allergies and respiratory infections in pediatric populations. Additionally, postbiotics—bioactive compounds from probiotic fermentation—offer promising benefits, such as improved gut barrier function, reduced inflammation, and enhanced nutrient absorption, while presenting fewer safety concerns compared to live probiotics. Conclusions: By examining the mechanisms of action and clinical evidence, this review underscores the potential of integrating probiotics and postbiotics into pediatric healthcare strategies to promote optimal growth, development, and overall health in children.</jats:p>
    Scopus© Citations 32
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    Phytochemical Composition and Biological Properties of Macleania rupestris Fruit Extract: Insights into Its Antimicrobial and Antioxidant Activity
    (MDPI AG, 2025-03-27)
    MAYORGA RAMOS, ARIANNA CAROLINA  
    ;
    ZUÑIGA MIRANDA, JOHANA JANINA  
    ;
    Elena Coyago-Cruz
    ;
    HEREDIA MOYA, JORGE HUMBERTO  
    ;
    Jéssica Guamán-Bautista
    Macleania rupestris, a fruit-bearing species of the Ericaceae family, has traditionally been used for its potential medicinal properties. Background/Objectives: This study investigates the phytochemical composition and antimicrobial activity of M. rupestris fruit extract, focusing on its antibacterial, antibiofilm, and antifungal effects. Methods: M. rupestris (Kunth) A.C.Sm. berries (code: 4456, Herbario QUPS-Ecuador) were collected from the cloud forest Montano Alto, Cuenca-Ecuador, and the extract was obtained using an ethanolic-based extraction and chemically characterized. The antibacterial and antifungal activity of the fruit extract was assessed against seven multidrug-resistant bacteria strains and four fungal strains using the microdilution method. The biofilm inhibition potential was evaluated using a microplate assay with the crystal violet staining method. The antioxidant activity was evaluated using DPPH and ABTS assays. Results: The bioactive compounds showed 853.9 mg phenols/100 g DW, 573.2 mg organic acid/100 g DW, and 21.5 mg C-3-gl/100 g DW of anthocyanins. The antibacterial assays demonstrated significant inhibitory activity against Enterococcus faecalis, Enterococcus faecium, Escherichia coli, and Staphylococcus epidermidis, with MIC values ranging from 1.25 to 5 mg/mL. Additionally, the biofilm inhibition assays confirmed the potential of M. rupestris extract to disrupt bacterial biofilms, particularly in S. aureus and L. monocytogenes. Nevertheless, no significant antifungal activity was observed against Candida spp., suggesting selective antimicrobial properties. Finally, the antioxidant activity was strong (1.62 mmol TE/100 g DW by DPPH and 3.28 mmol TE/100 g DW by ABTS). Conclusions: These findings indicate that M. rupestris possesses promising antibacterial, antibiofilm, and antioxidant properties, which may be attributed to its phenolic and organic acid composition. Further fractionation and targeted bioassays are required to elucidate the specific bioactive compounds responsible for these effects and explore their potential applications in antimicrobial formulations.
    Scopus© Citations 7
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    Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari
    (Springer Science and Business Media LLC, 2018-05-15)
    GUAMAN BAUTISTA, LINDA PRISCILA  
    ;
    Carlos Barba-Ostria
    ;
    Fuzhong Zhang
    ;
    Edmar R. Oliveira-Filho
    ;
    José Gregório C. Gomez
    Scopus© Citations 27
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    Tracking SARS-CoV-2: Novel Trends and Diagnostic Strategies
    (MDPI AG, 2021-10-26)
    GUAMAN BAUTISTA, LINDA PRISCILA  
    ;
    Erick Moreta-Urbano
    ;
    Claudia G. Oña-Arias
    ;
    Marbel Torres-Arias
    ;
    Nikolaos C. Kyriakidis
    <jats:p>The COVID-19 pandemic has had an enormous impact on economies and health systems globally, therefore a top priority is the development of increasingly better diagnostic and surveillance alternatives to slow down the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In order to establish massive testing and contact tracing policies, it is crucial to have a clear view of the diagnostic options available and their principal advantages and drawbacks. Although classical molecular methods such as RT-qPCR are broadly used, diagnostic alternatives based on technologies such as LAMP, antigen, serological testing, or the application of novel technologies such as CRISPR-Cas for diagnostics, are also discussed. The present review also discusses the most important automation strategies employed to increase testing capability. Several serological-based diagnostic kits are presented, as well as novel nanotechnology-based diagnostic methods. In summary, this review provides a clear diagnostic landscape of the most relevant tools to track COVID-19.</jats:p>
    Scopus© Citations 12
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    Scopus© Citations 321