GUÁMAN BAUTISTA, LINDA PRISCILA

Preferred name

Main Affiliation

Facultad de Ciencias de la Salud Eugenio Espejo

CENBIO - Centro de Investigación Biomédica

Web Site

Google Scholar

Scopus Author ID

57190735993

Now showing 1 - 10 of 27

Some of the metrics are blocked by your
consent settings
Advances in environmental biotechnology with CRISPR/Cas9: bibliometric review and cutting-edge applications
(Springer Science and Business Media LLC, 2025-02-22)
Jessica Verdezoto Prado
;
Cristhian Chicaiza Ortiz
;
Ana Belén Mejía Pérez
;
Camila Freire Torres
;
Marco Viteri Yánez
CRISPR/Cas9 has emerged as the predominant method for genome editing due to its cost-effectiveness and broad applicability, playing a crucial role in advancing sustainable practices across various sectors. This systematic review employs the PRISMA methodology to evaluate the impact of CRISPR/Cas9 on environmental protection and on achieving Sustainable Development Goals (SDGs) such as SDG 2, 3, 6, 7, 9, 12, 13, and SDG15. These goals focus on the responsible use of natural resources, reducing the negative effects of climate change and ensuring safe food for the entire population. Analyzing data from the Web of Science, the review found significant growth in related publications, with a 30% increase since 2014, predominantly from the US, China, Germany, and the UK. The study categorizes the scientific developments into these trends, the enhancement of plant tolerance to environmental stresses, as evidenced by the consistent focus on terms such as “tolerance” and “plant” since 2021. Furthermore, the relevance of “Gene Editing” has increased significantly since 2022, underscoring the importance of CRISPR/Cas9 in developing resilient crops that can withstand extreme conditions. These trends underscore the growing significance of biotechnological advancements in the mitigation of climate change’s effects and the improvement of ecosystem stability. Key discussions include CRISPR/Cas9’s role in the development of fourth-generation biofuels and environmental biosensors, as well as its applications in enhancing genetic resilience and controlling invasive species. These innovations highlight CRISPR/Cas9’s potential in promoting sustainable resource management and energy generation, making a significant contribution to ecological conservation and sustainability efforts.
Some of the metrics are blocked by your
consent settings
Bacteriophage-mediated approaches for biofilm control
(Frontiers Media SA, 2024-10-07)
MAYORGA RAMOS, ARIANNA CAROLINA
;
Saskya E. Carrera-Pacheco
;
Carlos Barba Ostria
;
GUAMAN BAUTISTA, LINDA PRISCILA
Biofilms are complex microbial communities in which planktonic and dormant bacteria are enveloped in extracellular polymeric substances (EPS) such as exopolysaccharides, proteins, lipids, and DNA. These multicellular structures present resistance to conventional antimicrobial treatments, including antibiotics. The formation of biofilms raises considerable concern in healthcare settings, biofilms can exacerbate infections in patients and compromise the integrity of medical devices employed during treatment. Similarly, certain bacterial species contribute to bulking, foaming, and biofilm development in water environments such as wastewater treatment plants, water reservoirs, and aquaculture facilities. Additionally, food production facilities provide ideal conditions for establishing bacterial biofilms, which can serve as reservoirs for foodborne pathogens. Efforts to combat antibiotic resistance involve exploring various strategies, including bacteriophage therapy. Research has been conducted on the effects of phages and their individual proteins to assess their potential for biofilm removal. However, challenges persist, prompting the examination of refined approaches such as drug-phage combination therapies, phage cocktails, and genetically modified phages for clinical applications. This review aims to highlight the progress regarding bacteriophage-based approaches for biofilm eradication in different settings.
Some of the metrics are blocked by your
consent settings
Crystal Structure, Hirshfeld Surface Analysis, and Biological Activities of Schiff-Base Derivatives of 4-Aminoantipyrine
(American Chemical Society (ACS), 2023-10-31)
Esteban Aguilar-Llanos
;
Saskya E. Carrera-Pacheco
;
GONZALEZ PASTOR, REBECA EUGENIA
;
Juan Carlos Romero-Benavides
;
Carlos Cevallos-Morillo
Some of the metrics are blocked by your
consent settings
Chemical Properties and Biological Activity of Bee Pollen
(MDPI AG, 2023-11-25)
Cristina Rodríguez-Pólit
;
GONZALEZ PASTOR, REBECA EUGENIA
;
Roberto Vallejo-Imbaquingo
;
Carlos Barba-Ostria
;
HEREDIA MOYA, JORGE HUMBERTO
<jats:p>Pollen, a remarkably versatile natural compound collected by bees for its abundant source of proteins and nutrients, represents a rich reservoir of diverse bioactive compounds with noteworthy chemical and therapeutic potential. Its extensive biological effects have been known and exploited since ancient times. Today, there is an increased interest in finding natural compounds against oxidative stress, a factor that contributes to various diseases. Recent research has unraveled a multitude of biological activities associated with bee pollen, ranging from antioxidant, anti-inflammatory, antimicrobial, and antifungal properties to potential antiviral and anticancer applications. Comprehending the extensive repertoire of biological properties across various pollen sources remains challenging. By investigating a spectrum of pollen types and their chemical composition, this review produces an updated analysis of the bioactive constituents and the therapeutic prospects they offer. This review emphasizes the necessity for further exploration and standardization of diverse pollen sources and bioactive compounds that could contribute to the development of innovative therapies.</jats:p>
Some of the metrics are blocked by your
consent settings
Mxenes as a versatile nanoplatform: Synthesis and emerging biomedical applications
(Elsevier BV, 2025-09-25)
Ali Mohammad Amani
;
Ehsan Vafa
;
Maryam Mirzae
;
Milad Abbasi
;
Ahmad Vaez
Recently, MXenes have garnered significant attention in academic research due to their remarkable structural, electrical, magnetic, optical, mechanical, and chemical properties. New advancements and emerging findings indicate that MXene, classified as a two-dimensional (2D) layered material, exhibits significantly more potential in the field of biomedicine and biotechnology compared to existing nanosystems. These applications include acting as antibacterial agents, biosensor systems, the delivery and loading of drugs, bioimaging, and therapeutic interventions. The unique characteristics of MXenes, such as their significant electrical conductivity, large surface area, low toxicity, magnetism, luminescence, and high biocompatibility, make them highly promising candidates for diagnostic, therapeutic, and theranostic applications. Surface modifications of MXenes exhibit biocompatibility and have multifunctional functions, including the ability to direct ligands towards specific spots for preferential aggregation, hence enabling their utilization in specialized applications. This paper provides an overview of the characteristics, modifications, and synthesis methods of MXene nanomaterials. The present article also delivers a comprehensive assessment of the practical uses of MXene-based nanomaterials in biomedicine, with a particular focus on biosensing, bioimaging, antibacterial effects, implants, and multifunctional therapeutic platforms. This paper also presents a discussion of the future prospects and challenges associated with the applications of MXenes in the biomedicine field.
Some of the metrics are blocked by your
consent settings
Phytosynthesis of Silver Nanoparticles Using Mansoa alliacea (Lam.) A.H. Gentry (Bignoniaceae) Leaf Extract: Characterization and Their Biological Activities
(MDPI AG, 2024-09-25)
ZUÑIGA MIRANDA, JOHANA JANINA
;
Saskya E. Carrera-Pacheco
;
GONZALEZ PASTOR, REBECA EUGENIA
;
MAYORGA RAMOS, ARIANNA CAROLINA
;
Cristina Rodríguez-Pólit
Background. Mansoa alliacea is a native plant renowned for its medicinal properties in traditional healing in the Amazon Region. This plant is rich in polyphenols, flavonoids, anthocyanins, phenolic acids, tannins, ketones, triterpenes, as well as other bioactive compounds. Objectives. This study aims to develop an innovative, eco-friendly method for synthesizing silver nanoparticles using an aqueous extract of M. alliacea (Ma-AgNPs), enhancing the biological activities of AgNPs by leveraging the therapeutic potential of the plant’s bioactive compounds. Methods. Silver nanoparticles were synthesized using the aqueous extract of M. alliacea. The biological activities of Ma-AgNPs were assessed, including antibacterial, anti-inflammatory, antioxidant, antitumor, and anti-biofilm effects, along with evaluating their hemolytic activity. Results. Quantitative analysis revealed that Ma-AgNPs exhibit potent antibacterial activity against multidrug and non-multidrug-resistant bacteria, with MIC values ranging from 1.3 to 10.0 µg/mL. The Ma-AgNPs significantly reduced NO production by 86.9% at 4 µg/mL, indicating strong anti-inflammatory effects. They demonstrated robust antioxidant activity with an IC50 of 5.54 ± 1.48 µg/mL and minimal hemolytic activity, with no hemolysis observed up to 20 µg/mL and only 4.5% at 40 µg/mL. Their antitumor properties were notable, with IC50 values between 2.9 and 5.4 µg/mL across various cell lines, and they achieved over 50% biofilm inhibition at concentrations of 30–40 µg/mL. Conclusions. These findings underscore the potential of Ma-AgNPs for biomedical applications, particularly in developing new antimicrobial agents and bioactive coatings with reduced toxicity. This research highlights a sustainable approach that not only preserves but also amplifies the inherent biological activities of plant extracts, paving the way for innovative therapeutic solutions.
Some of the metrics are blocked by your
consent settings
Bioactive Phenolic Compounds from Rambutan - Nephelium lappaceum L. - Shell: Encapsulation, Structural Stability, and Multifunctional Activities
(MDPI AG, 2025-11-09)
Carlos Barba Ostria
;
Orestes López
;
Alexis Debut
;
MAYORGA RAMOS, ARIANNA CAROLINA
;
ZÚÑIGA MIRANDA, JOHANA JANINA
Rambutan (Nephelium lappaceum) shell, an agro-industrial by-product, is a rich source of phenolic acids and minor anthocyanins, but its direct use is limited by instability and low bioavailability. We extracted phenolic-rich fractions and produced maltodextrin microcapsules by spray drying, then confirmed chemical entrapment and amorphization by FTIR, SEM, and XRD. The formulation showed high encapsulation efficiency and high antioxidant capacity (DPPH), selective bactericidal activity against Pseudomonas aeruginosa and Burkholderia cepacia, and strong inhibition of Staphylococcus aureus and Listeria monocytogenes biofilms, while exhibiting negligible hemolysis (<2%) across tested concentrations. Antitumor effects were moderate with low selectivity in vitro, indicating that phenolic-acid-driven redox modulation may require fractionation or delivery optimization for oncology applications. Overall, spray-dried microcapsules provided structural stability and safety while concentrating multifunctional activities relevant to food and biomedical uses. By valorizing a tropical waste stream into a bioactive, hemocompatible ingredient, this work aligns with societal goals on health and sustainable production (SDG 3 and SDG 12) and offers a scalable route to deploy underutilized phenolic resources.</jats:p>
Some of the metrics are blocked by your
consent settings
Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira
(Springer Science and Business Media LLC, 2019-02-12)
L. G. V. Fernandes
;
GUAMAN BAUTISTA, LINDA PRISCILA
;
S. A. Vasconcellos
;
Marcos B. Heinemann
;
M. Picardeau
<jats:title>Abstract</jats:title><jats:p>Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus <jats:italic>Leptospira</jats:italic>, which also includes free-living saprophyte strains. Many aspects of leptospiral basic biology and virulence mechanisms remain unexplored mainly due to the lack of effective genetic tools available for these bacteria. Recently, the type II CRISPR/Cas system from <jats:italic>Streptococcus pyogenes</jats:italic> has been widely used as an efficient genome engineering tool in bacteria by inducing double-strand breaks (DSBs) in the desired genomic targets caused by an RNA-guided DNA endonuclease called Cas9, and the DSB repair associated machinery. In the present work, plasmids expressing heterologous <jats:italic>S. pyogenes</jats:italic> Cas9 in <jats:italic>L. biflexa</jats:italic> cells were generated, and the enzyme could be expressed with no apparent toxicity to leptospiral cells. However, <jats:italic>L. biflexa</jats:italic> cells were unable to repair RNA-guided Cas9-induced DSBs. Thus, we used a catalytically dead Cas9 (dCas9) to obtain gene silencing rather than disruption, in a strategy called CRISPR interference (CRISPRi). We demonstrated complete gene silencing in <jats:italic>L. biflexa</jats:italic> cells when both dCas9 and single-guide RNA (sgRNA) targeting the coding strand of the β-galactosidase gene were expressed simultaneously. Furthermore, when the system was applied for silencing the <jats:italic>dnaK</jats:italic> gene, no colonies were recovered, indicating that DnaK protein is essential in <jats:italic>Leptospira</jats:italic>. In addition, flagellar motor switch FliG gene silencing resulted in reduced bacterial motility. To the best of our knowledge, this is the first work applying the CRISPRi system in <jats:italic>Leptospira</jats:italic> and spirochetes in general, expanding the tools available for understanding leptospiral biology.</jats:p>
Some of the metrics are blocked by your
consent settings
Clinical, molecular, and epidemiological characterization of the SARS-CoV-2 virus and the Coronavirus Disease 2019 (COVID-19), a comprehensive literature review
(Elsevier BV, 2020-09)
Esteban Ortiz-Prado
;
Katherine Simbaña-Rivera
;
Lenin Gómez- Barreno
;
Mario Rubio-Neira
;
GUAMAN BAUTISTA, LINDA PRISCILA
Some of the metrics are blocked by your
consent settings
Phytochemical Composition and Biological Properties of Macleania rupestris Fruit Extract: Insights into Its Antimicrobial and Antioxidant Activity
(MDPI AG, 2025-03-27)
MAYORGA RAMOS, ARIANNA CAROLINA
;
ZUÑIGA MIRANDA, JOHANA JANINA
;
Elena Coyago-Cruz
;
HEREDIA MOYA, JORGE HUMBERTO
;
Jéssica Guamán-Bautista
Macleania rupestris, a fruit-bearing species of the Ericaceae family, has traditionally been used for its potential medicinal properties. Background/Objectives: This study investigates the phytochemical composition and antimicrobial activity of M. rupestris fruit extract, focusing on its antibacterial, antibiofilm, and antifungal effects. Methods: M. rupestris (Kunth) A.C.Sm. berries (code: 4456, Herbario QUPS-Ecuador) were collected from the cloud forest Montano Alto, Cuenca-Ecuador, and the extract was obtained using an ethanolic-based extraction and chemically characterized. The antibacterial and antifungal activity of the fruit extract was assessed against seven multidrug-resistant bacteria strains and four fungal strains using the microdilution method. The biofilm inhibition potential was evaluated using a microplate assay with the crystal violet staining method. The antioxidant activity was evaluated using DPPH and ABTS assays. Results: The bioactive compounds showed 853.9 mg phenols/100 g DW, 573.2 mg organic acid/100 g DW, and 21.5 mg C-3-gl/100 g DW of anthocyanins. The antibacterial assays demonstrated significant inhibitory activity against Enterococcus faecalis, Enterococcus faecium, Escherichia coli, and Staphylococcus epidermidis, with MIC values ranging from 1.25 to 5 mg/mL. Additionally, the biofilm inhibition assays confirmed the potential of M. rupestris extract to disrupt bacterial biofilms, particularly in S. aureus and L. monocytogenes. Nevertheless, no significant antifungal activity was observed against Candida spp., suggesting selective antimicrobial properties. Finally, the antioxidant activity was strong (1.62 mmol TE/100 g DW by DPPH and 3.28 mmol TE/100 g DW by ABTS). Conclusions: These findings indicate that M. rupestris possesses promising antibacterial, antibiofilm, and antioxidant properties, which may be attributed to its phenolic and organic acid composition. Further fractionation and targeted bioassays are required to elucidate the specific bioactive compounds responsible for these effects and explore their potential applications in antimicrobial formulations.

GUÁMAN BAUTISTA, LINDA PRISCILA

Filters

Settings

Sort By

Results per page