FLORES FLOR, FRANCISCO JAVIER

Preferred name

Main Affiliation

Facultad de Ciencias de la Ingeniería e Industrias

CIAL - Centro de Investigación de Alimentos

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ORCID

0000-0001-8092-3750

Scopus Author ID

58620465200

Now showing 1 - 10 of 37

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Phylodynamics and Coat Protein Analysis of Babaco Mosaic Virus in Ecuador
(MDPI AG, 2022-06-22)
Francisco Mosquera-Yuqui
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FLORES FLOR, FRANCISCO JAVIER
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Eduardo A. Moncayo
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Brighitte A. Garzón-Proaño
;
Miguel A. Méndez
<jats:p>Babaco is a fast-growing herbaceous shrub with great commercial potential because of the organoleptic properties of its fruit. Babaco mosaic virus (BabMV) is a potexvirus in the family Alphaflexiviridae affecting babaco in all the provinces that produce this crop in Ecuador. BabMV was recently described but it has been affecting babaco for decades and, since many potexviruses are serologically indistinguishable, it may have been previously misidentified as papaya mosaic virus. Based on the coat protein (CP) gene, we aimed to study the distribution and epidemiological patterns of BabMV in babaco and chamburo over the years and to model its three-dimensional structure. Sequences of the CP were obtained from thirty-six isolates from plants collected in the main babaco-producing provinces of Ecuador between 2016 and 2021. The evolution rate of BabMV was estimated at 1.21 × 10−3 nucleotide substitutions site−1 year−1 and a time of origin of the most recent common ancestor around 1958.80. From molecular dynamics simulations, compared to other proteins of BabMV—RDRP, TGB1, and Alkb domain—the CP exhibited a higher flexibility with the C and N terminals as the most flexible regions. The reconstructed viral distribution provides dispersion patterns which have implications for control approaches of BabMV.</jats:p>
Scopus© Citations 6
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Identification and Characterization ofPythium,Globisporangium, andPhytopythiumSpecies Present in Floricultural Crops from Long Island, New York
(Scientific Societies, 2023-07)
Patricia A. Garrido
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Fernanda Proaño-Cuenca
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FLORES FLOR, FRANCISCO JAVIER
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Edinson A. Díaz Benítez
;
Ivanna F. Sánchez Torres
<jats:p>Several Pythium, Globisporangium, and Phytopythium species cause Pythium diseases in greenhouse floricultural crops, resulting in significant seasonal losses. Four hundred and eighteen Pythium, Globisporangium, and Phytopythium isolates from flowering crops, growing media, or bench and floor debris were collected from Long Island greenhouses or clinic samples between 2002 and 2013. Isolates were identified to species based on morphology and internal transcribed spacer barcoding. Twenty-two species of Pythium, Phytopythium, and Globisporangium were identified, with Globisporangium irregulare sensu lato (s.l.) being the most common. To determine the origin of inoculum during the 2011 cropping season, 11 microsatellite loci were analyzed in 124 G. irregulare s.l. isolates collected in four greenhouses and six previously collected from clinic samples. Cluster analyses grouped G. irregulare s.l. isolates into four groups: G. irregulare sensu stricto, plus three G. cryptoirregulare clusters. The population structure defined by greenhouse and host was found in two clades. Additionally, the population dynamics of G. irregulare s.l. isolates associated with Pelargonium spp. from 2011 to 2013 were examined using 85 isolates and nine informative microsatellite loci to assess inoculum survival over multiple cropping seasons. Although most isolates had unique genotypes, closely related genotypes were found in the same locations over different years. Our results indicate that G. irregulare s.l. inocula have local as well as remote origins. Isolates may be initially brought into ornamental operations from common sources, such as infected plant materials or infested potting mixes. Our results support the hypothesis that established strains can serve as inocula and survive in greenhouse facilities over multiple seasons.</jats:p>
Scopus© Citations 4
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Botrytis cinerea as a cause of blackberry gray mold disease in Ecuador
(Springer Science and Business Media LLC, 2024-06-18)
I. E. Jinez
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FLORES FLOR, FRANCISCO JAVIER
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Exploring the virome of Vasconcellea x heilbornii: the first step towards a sustainable production program for babaco in Ecuador
(Springer Science and Business Media LLC, 2020-06-16)
Juan F. Cornejo-Franco
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Andrés Medina-Salguero
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FLORES FLOR, FRANCISCO JAVIER
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Eduardo Chica
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Samuel Grinstead
Scopus© Citations 18
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First Report of Dickeya fangzhongdai Causing Soft Rot in Bananas in Ecuador
(Scientific Societies, 2025-03-12)
A. Toaza
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FLORES FLOR, FRANCISCO JAVIER
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R. B. Caiza
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P. A. Garrido
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A. D. Salazar
Scopus© Citations 1
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First Report of Pantoea ananatis Causing Leaf Spot Disease of Maize in Ecuador
(Scientific Societies, 2021-10-01)
A. Toaza
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R. B. Caiza
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A. D. Garrido
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C. R. Moreno
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J. Guevara
Scopus© Citations 7
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Batch mesophilic anaerobic digestion of mixtures of lignocellulosic biomasses from Ecuador: influence of inoculum type and thermal pretreatment on methane production and process kinetics
(Springer Science and Business Media LLC, 2025-04-21)
O. H. López-Balladares
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D. De la Lama-Calvente
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FLORES FLOR, FRANCISCO JAVIER
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R. Borja
Comprehensive and integral treatment of agricultural wastes is crucial to palliate the environmental impact associated with uncontrolled disposal. However, waste management should be focused on a local perspective. In this study, the anaerobic co-digestion of four lignocellulosic by-products generated in Ecuador (cocoa husks, coconut shells, rice hulls, and sugarcane bagasse) was evaluated. Two different mixture ratios, the impact of thermal pretreatment, and the influence of the inoculum origin were also assessed. Results showed that the anaerobic inoculum collected from a brewery factory showed the highest efficiency. Moreover, thermal pretreatment improved the methane yields (158–160 NLCH4 kg−1VS). However, the highest kinetic constant (1.1 ± 0.1 d−1; first-order kinetic model) and the maximum methane production rate (153 ± 3 NLCH4 kg−1VS d−1; logistic model) were obtained for the untreated mixtures. Finally, the substrate mixture ratio did not influence the anaerobic digestion performance. Therefore, the present study provides preliminary insights into the viability of anaerobic co-digestion as a feasible technology for the valorization of agro-industrial lignocellulosic residues to reduce the effect of seasonal production variations.
Scopus© Citations 4
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Salmonella enterica Serovar Infantis KPC-2 Producer: First Isolate Reported in Ecuador
(Mary Ann Liebert Inc, 2024-12-01)
VILLAVICENCIO ZAMBRANO, FERNANDO XAVIER
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Viviana Albán
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Carolina Satán
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Hidaleisy Quintana
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Wladimir Enríquez
Antimicrobial resistance is currently considered a public health threat. Carbapenems are antimicrobials for hospital use, and Enterobacterales resistant to these β-lactams have spread alarmingly in recent years, especially those that cause health care-associated infections. The blaKPC gene is considered one of the most important genetic determinants disseminated by plasmids, promoting horizontal gene transfer. This study describes, for the first time in Ecuador, and worldwide, the presence of a blaKPC-2 gene in an isolate of Salmonella enterica serovar Infantis from a clinical sample. Through whole-genome sequencing, we characterized the genetic determinants of antimicrobial resistance in this Salmonella ST-32 strain. Our results showed the presence of several resistance genes, including blaCTX-M-65, and a conjugative plasmid Kpn-WC17-007-03 that may be responsible for the horizontal transference of these resistance mechanisms.
Scopus© Citations 1
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Phylodynamics of classical swine fever virus with emphasis on Ecuadorian strains
(Hindawi Limited, 2018-01-10)
A. D. Garrido
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M. Barrera Valle
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A. Acosta
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FLORES FLOR, FRANCISCO JAVIER
Scopus© Citations 15
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Integrating mathematical modeling into synthetic biology education: a comprehensive approach through webinars and syllabus development
(Frontiers Media SA, 2024-10-18)
Yadira Boada
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FLORES FLOR, FRANCISCO JAVIER
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Alejandro Vignoni
Mathematical modeling is a fundamental aspect of synthetic biology, enabling precise design and analysis of biological systems. To enhance students' understanding of this critical topic, we developed a series of webinars aimed at teaching mathematical modeling to iGEM teams. These webinars were initially created to maintain student engagement during a period of restricted lab access but quickly demonstrated their value as an effective educational tool. The success of these webinars highlighted the suitability of mathematical modeling as a topic well-suited to both onsite and online learning environments. Recognizing this, we expanded the content into a comprehensive syllabus for undergraduate courses in synthetic biology at the Universitat Politècnica de Valencia in Spain and Universidad de las Fuerzas Armadas—ESPE in Ecuador. The course now serves as a core component of synthetic biology education, offering students a robust framework for understanding and applying mathematical models. It includes a series of lectures, practical exercises, and case studies, all designed to deepen students' knowledge and skills in this essential area. To support educators and students, we have also developed a deck of slides and example scripts that provide practical examples and reinforce the concepts taught in the course. This manuscript presents the development, implementation, and impact of these educational initiatives, demonstrating how mathematical modeling can be effectively integrated into synthetic biology curricula to prepare students for real-world challenges in the field.

FLORES FLOR, FRANCISCO JAVIER

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