FLORES FLOR, FRANCISCO JAVIER
Preferred name
FLORES FLOR, FRANCISCO JAVIER
Main Affiliation
CIAL - Centro de Investigación de Alimentos
Web Site
ORCID
0000-0001-8092-3750
Scopus Author ID
58620465200
37 results
Now showing 1 - 10 of 37
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Item type:Publication, Phylodynamics of classical swine fever virus with emphasis on Ecuadorian strains(Hindawi Limited, 2018-01-10)Scopus© Citations 15 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Batch mesophilic anaerobic digestion of mixtures of lignocellulosic biomasses from Ecuador: influence of inoculum type and thermal pretreatment on methane production and process kinetics(Springer Science and Business Media LLC, 2025-04-21); R. BorjaComprehensive and integral treatment of agricultural wastes is crucial to palliate the environmental impact associated with uncontrolled disposal. However, waste management should be focused on a local perspective. In this study, the anaerobic co-digestion of four lignocellulosic by-products generated in Ecuador (cocoa husks, coconut shells, rice hulls, and sugarcane bagasse) was evaluated. Two different mixture ratios, the impact of thermal pretreatment, and the influence of the inoculum origin were also assessed. Results showed that the anaerobic inoculum collected from a brewery factory showed the highest efficiency. Moreover, thermal pretreatment improved the methane yields (158–160 NLCH4 kg−1VS). However, the highest kinetic constant (1.1 ± 0.1 d−1; first-order kinetic model) and the maximum methane production rate (153 ± 3 NLCH4 kg−1VS d−1; logistic model) were obtained for the untreated mixtures. Finally, the substrate mixture ratio did not influence the anaerobic digestion performance. Therefore, the present study provides preliminary insights into the viability of anaerobic co-digestion as a feasible technology for the valorization of agro-industrial lignocellulosic residues to reduce the effect of seasonal production variations.Scopus© Citations 4 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of Dickeya fangzhongdai Causing Soft Rot in Bananas in Ecuador(Scientific Societies, 2025-03-12); Scopus© Citations 1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Exploring the virome of Vasconcellea x heilbornii: the first step towards a sustainable production program for babaco in Ecuador(Springer Science and Business Media LLC, 2020-06-16); Scopus© Citations 18 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of <i>Pantoea ananatis</i> Causing Leaf Spot Disease of Maize in Ecuador(Scientific Societies, 2021-10-01)Scopus© Citations 7 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Integrating mathematical modeling into synthetic biology education: a comprehensive approach through webinars and syllabus developmentMathematical modeling is a fundamental aspect of synthetic biology, enabling precise design and analysis of biological systems. To enhance students' understanding of this critical topic, we developed a series of webinars aimed at teaching mathematical modeling to iGEM teams. These webinars were initially created to maintain student engagement during a period of restricted lab access but quickly demonstrated their value as an effective educational tool. The success of these webinars highlighted the suitability of mathematical modeling as a topic well-suited to both onsite and online learning environments. Recognizing this, we expanded the content into a comprehensive syllabus for undergraduate courses in synthetic biology at the Universitat Politècnica de Valencia in Spain and Universidad de las Fuerzas Armadas—ESPE in Ecuador. The course now serves as a core component of synthetic biology education, offering students a robust framework for understanding and applying mathematical models. It includes a series of lectures, practical exercises, and case studies, all designed to deepen students' knowledge and skills in this essential area. To support educators and students, we have also developed a deck of slides and example scripts that provide practical examples and reinforce the concepts taught in the course. This manuscript presents the development, implementation, and impact of these educational initiatives, demonstrating how mathematical modeling can be effectively integrated into synthetic biology curricula to prepare students for real-world challenges in the field. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of Strawberry Wilt Caused by <i>Fusarium oxysporum</i> in Ecuador(Scientific Societies, 2020-05)Scopus© Citations 1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Complete Genome Sequence of
<i>Alstroemeria</i>
<i>Necrotic Streak Virus</i>
Isolated from Limonium sinuatum in Ecuador(American Society for Microbiology, 2022-11-17); Simon Roux<jats:p> The complete genome sequence of <jats:italic>Alstroemeria</jats:italic> <jats:italic>necrotic streak virus</jats:italic> (ANSV) isolated from the statice cut flower ( <jats:named-content content-type="genus-species">Limonium sinuatum</jats:named-content> ) is presented. The genome has the three characteristic segments of the genus <jats:italic>Orthotospovirus</jats:italic> , family <jats:italic>Tospoviridae</jats:italic> , the S segment (3,135 bp), the M segment (4,869 bp), and the L segment (8,755 bp). </jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Complete genome sequence and phylogenetic analysis of a newly discovered fusagra-like virus infecting Carica papaya in Ecuador(Springer Science and Business Media LLC, 2024-06-20); Diego F. Quito-AvilaA new fusagra-like virus infecting papaya (Carica papaya L.) was genetically characterized. The genome of the virus, provisionally named "papaya sticky fruit-associated virus" (PSFaV), is a single molecule of double-stranded RNA, 9,199 nucleotides (nt) in length, containing two discontinuous open reading frames. Pairwise sequence comparisons based on complete RNA-dependent-RNA-polymerase (RdRp) sequences revealed identity of 79.4% and 83.3% at the nt and amino acid (aa) level, respectively, to babaco meleira-like virus (BabMelV), an uncharacterized virus sequence discovered in babaco (Vasconcellea x heilbornii) in Ecuador. Additional plant-associated viruses with sequence identity in the 50% range included papaya meleira virus (PMeV) isolates from Brazil. Phylogenetic analysis based on the amino acid sequences of the capsid protein (CP), RdRp, and CP-RdRp fusion protein genes placed PSFaV in a group within a well-supported clade that shares a recent ancestor with Sclerotium rolfsii RNA virus 2 and Phlebiopsis gigantea mycovirus dsRNA 2, two fungus-associated fusagraviruses. Genomic features and phylogenetic relatedness suggest that PSFaV, along with its closest relative BabMelV, represent a species of novel plant-associated virus classified within the recently established family Fusagraviridae.Scopus© Citations 2 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of Pectobacterium brasiliense Causing Banana Soft Rot in Ecuador(Scientific Societies, 2024-02-01); L. A. RamosBanana (Musa spp.) is the most economically important crop in Ecuador, with exports representing 35% of the agricultural GDP of the country. It covers 230,000 ha, mostly concentrated in three coastal provinces, Guayas, Los Ríos, and El Oro. Between July and September 2022, disease symptomatic banana cultivar Williams plants were observed in commercial plantations located in two parishes in the province of Guayas (Naranjito and Lorenzo de Garaicoa) and one parish in the province of Santo Domingo de los Tsáchilas (La Concordia), with an incidence that ranged from 5 to 15%. Symptoms included soft rot of the pseudostem and rhizome decay, characterized by a fetid odor. Three symptomatic pseudostems from each location were collected, washed with running water to remove any debris, and dried with absorbent paper. From the lesion of each pseudostem, seven pieces of 2 cm2 were taken, surface sterilized, and macerated in 9 ml of sterile peptone water (0.1% w/v). The macerate was diluted threefold in sterile water, plated on nutrient agar, and incubated at 30°C for 24 h. Eight randomly picked colonies, with convex elevation and creamy white color, were isolated on nutrient agar. Each of the bacterial isolates was biochemically profiled by the Biolog system (Biolog, U.S.A.) and identified as Pectobacterium. Three isolates, one from each parish (FP220416, FP220694, and FP220904), were selected for testing Koch’s postulates and further identification. Sequences from fragments of the 16S, dnaA, gapA, and gyrB genes were obtained from these isolates, following the protocols used by Dobhal et al. (2020) and Boluk et al. (2020), showing 98.1 to 99.0%, 98.2%, 99.7 to 99.8%, and 98.4 to 98.9% identity, respectively, with sequences from the Pectobacterium brasiliense type strain LMG_21371 (accession no. JQOE00000000). The obtained sequences were deposited in GenBank with the following accession numbers: OR392417, OR371545, OR371546, OR727281, OR727282, and OR739074 to OR739080. Using BEAST version 1.10.4 (Suchard et al. 2018), a Bayesian multilocus phylogenetic tree was built with multiple sequence alignments of dnaA, gapA, and gyrB from 22 P. brasiliense isolates and two P. aquaticum isolates used as outgroup. The phylogenetic analysis showed that the Ecuadorian isolates cluster with P. brasiliense BF20 isolated from Opuntia ficus-indica in Mexico and are closely related with the type strain. Pathogenicity tests were conducted through syringe infiltration with 1 ml of 1 × 108 CFU/ml bacterial suspension. Each of the three characterized isolates were inoculated into the pseudostems of five healthy 4-month-old banana plants of the cultivar Williams. Negative control plants were infiltrated with sterile distilled water. The plants were incubated at 25°C and 74% relative humidity. Black lesions started to appear 11 days after inoculation, and 5 weeks after inoculation plants showed clear symptoms of soft rot of the pseudostem, including fetid odor associated with plant tissue decomposition. The control plants remained symptom-free. Bacteria were reisolated only from symptomatic pseudostems and identified as P. brasiliense with the specific primers Pb1F and Pb1R.Scopus© Citations 1