FLORES FLOR, FRANCISCO JAVIER
Preferred name
FLORES FLOR, FRANCISCO JAVIER
Main Affiliation
CIAL - Centro de Investigación de Alimentos
Web Site
ORCID
0000-0001-8092-3750
Scopus Author ID
58620465200
37 results
Now showing 1 - 10 of 37
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Item type:Publication, First Report of <i>Pantoea ananatis</i> Causing Leaf Spot Disease of Maize in Ecuador(Scientific Societies, 2021-10-01) ;A. Toaza ;R. B. Caiza ;A. D. Garrido ;C. R. MorenoJ. Guevara - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Current Research on Andean Fruit Crop Diseases(Springer International Publishing, 2020) ;Erika Benítez ;William Viera; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Draft Genome Sequence of a Metronidazole-Resistant Bacteroides fragilis Strain Isolated in Ecuador(American Society for Microbiology, 2019-12-05) ;Jeannete Zurita ;Gabriela Sevillano ;Ariane Paz y Miño; Marcela Bovera<jats:p> Here, we report the draft genome sequence of <jats:named-content content-type="genus-species">Bacteroides fragilis</jats:named-content> strain Z&Z143, a metronidazole-resistant bacterium isolated from a blood culture from an Ecuadorian patient hospitalized in a medical institution in Quito, Ecuador. We describe a new variant of the <jats:italic>nim</jats:italic> genes, which is associated with metronidazole resistance. </jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Multiplex End-Point PCR for the Detection of Three Species of <i>Ophiosphaerella</i> Causing Spring Dead Spot of Bermudagrass(Scientific Societies, 2019-08) ;J. Francisco Iturralde Martinez; ;Alma R. Koch Kaiser ;Carla D. GarzónNathan R. Walker<jats:p> A multiplex end-point polymerase chain reaction (PCR) assay was developed for identifying the three-fungal species in the genus Ophiosphaerella that cause spring dead spot (SDS), a devastating disease of bermudagrass. These fungi are difficult to identify by morphology because they seldom produce pseudothecia. To achieve species-specific diagnosis, three pairs of primers were designed to identify fungal isolates and detect the pathogen in infected roots. The internal transcribed spacer region, the translation elongation factor 1-α, and the RNA polymerase II second-largest subunit were selected as targets and served as templates for the design of each primer pair. To achieve uniform melting temperatures, three to five random nucleotide extensions (flaps) were added to the 5′ terminus of some of the designed specific primers. Temperature cycling conditions and PCR components were standardized to optimize specificity and sensitivity of the multiplex reaction. Primers were tested in multiplex on DNA extracted from axenic fungal cultures and from field-collected infected and uninfected roots. A distinct amplicon was produced for each Ophiosphaerella sp. tested. The DNA from Ophiosphaerella close relatives and other common bermudagrass pathogens did not amplify during the multiplex assay. Metagenomic DNA from infected bermudagrass produced species-specific amplicons while DNA extracted from noninfected roots did not. This multiplex end-point PCR approach is a sensitive and specific molecular technique that allows for correct identification of SDS-associated Ophiosphaerella spp. from field-collected roots. </jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Salmonella enterica Serovar Infantis KPC-2 Producer: First Isolate Reported in Ecuador(Mary Ann Liebert Inc, 2024-12-01); ;Viviana Albán ;Carolina Satán ;Hidaleisy QuintanaWladimir EnríquezAntimicrobial resistance is currently considered a public health threat. Carbapenems are antimicrobials for hospital use, and Enterobacterales resistant to these β-lactams have spread alarmingly in recent years, especially those that cause health care-associated infections. The blaKPC gene is considered one of the most important genetic determinants disseminated by plasmids, promoting horizontal gene transfer. This study describes, for the first time in Ecuador, and worldwide, the presence of a blaKPC-2 gene in an isolate of Salmonella enterica serovar Infantis from a clinical sample. Through whole-genome sequencing, we characterized the genetic determinants of antimicrobial resistance in this Salmonella ST-32 strain. Our results showed the presence of several resistance genes, including blaCTX-M-65, and a conjugative plasmid Kpn-WC17-007-03 that may be responsible for the horizontal transference of these resistance mechanisms. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Integrating mathematical modeling into synthetic biology education: a comprehensive approach through webinars and syllabus developmentMathematical modeling is a fundamental aspect of synthetic biology, enabling precise design and analysis of biological systems. To enhance students' understanding of this critical topic, we developed a series of webinars aimed at teaching mathematical modeling to iGEM teams. These webinars were initially created to maintain student engagement during a period of restricted lab access but quickly demonstrated their value as an effective educational tool. The success of these webinars highlighted the suitability of mathematical modeling as a topic well-suited to both onsite and online learning environments. Recognizing this, we expanded the content into a comprehensive syllabus for undergraduate courses in synthetic biology at the Universitat Politècnica de Valencia in Spain and Universidad de las Fuerzas Armadas—ESPE in Ecuador. The course now serves as a core component of synthetic biology education, offering students a robust framework for understanding and applying mathematical models. It includes a series of lectures, practical exercises, and case studies, all designed to deepen students' knowledge and skills in this essential area. To support educators and students, we have also developed a deck of slides and example scripts that provide practical examples and reinforce the concepts taught in the course. This manuscript presents the development, implementation, and impact of these educational initiatives, demonstrating how mathematical modeling can be effectively integrated into synthetic biology curricula to prepare students for real-world challenges in the field. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Dactylonectria and Ilyonectria Species Causing Black Foot Disease of Andean Blackberry (Rubus Glaucus Benth) in Ecuador(MDPI AG, 2019-11-14) ;Jessica Sánchez ;Paola Iturralde ;Alma Koch ;Cristina TelloDennis Martinez<jats:p>Andean blackberry (Rubus glaucus Benth) plants from the provinces of Tungurahua and Bolivar (Ecuador) started showing symptoms of black foot disease since 2010. Wilted plants were sampled in both provinces from 2014 to 2017, and fungal isolates were obtained from tissues surrounding necrotic lesions in the cortex of the roots and crown. Based on morphological characteristics and DNA sequencing of histone 3 and the translation elongation factor 1α gene, isolates were identified as one of seven species, Ilyonectria vredehoekensis, Ilyonectria robusta, Ilyonectria venezuelensis, Ilyonectria europaea, Dactylonectria torresensis, or Dactylonectria novozelandica. Pathogenicity tests with isolates from each species, excluding I. europaea and D. novozelandica whose isolates were lost due to contamination, confirmed that the four species tested can produce black foot disease symptoms in Andean blackberry. This is the first report of Dactylonectria and Ilyonectria species causing black foot disease of Andean blackberry.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Characterization and genome analysis of Cucumber mosaic virus on commercial tobacco plants in Ecuador(Springer Science and Business Media LLC, 2024-04-02) ;Enny Ganchozo-Mendoza ;Ayane F. Ferreira Quadros ;Francisco Murilo Zerbini; Felipe R. Garcés-FiallosTobacco (Nicotiana tabacum L.), an endemic species of South America that is grown worldwide, is a member of the Solanaceae family. Although it is not a common crop in Ecuador, tobacco is important to the nation's economy and a significant source of employment. Viral infections are particularly devastating to tobacco as they can reduce both its yield and quality. In July 2021, symptoms of chlorosis, mosaic and leaf distortion were observed in four commercial fields in the provinces of Guayas and Los Ríos in Ecuador. This study aims to comprehensively characterize a viral isolate obtained from symptomatic plants. Using high throughput sequencing (HTS), phylogenetic analysis of the different open reading frames, and transmission electron microscopy (TEM) of symptomatic plants, we report for the first time the molecular characterization and genome sequence of Cucumber mosaic virus (CMV) isolated from commercial tobacco plants in Ecuador. The ability to detect and identify CMV in tobacco commercial plant fields enables the development of efficient control strategies, thereby mitigating constraints on the production of high-quality tobacco leaves. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genetic diversity and organic waste degrading capacity of <i>Hermetia illucens</i> from the evergreen forest of the Equatorial Choco lowland<jats:p>Globally, microplastics (MP) represent a growing burden for ecosystems due to their increasing presence at different trophic levels. In Ecuador, the lack of waste segregation has increased the quantity of waste, primarily organics and plastics, overloading landfills and water sources. Over time, plastics reduce in size and silently enter the food chain of animals, such as insects. The black soldier fly (BSF) larvae, <jats:italic>Hermetia illucens</jats:italic> (Linnaeus, 1758), is a species with devouring behavior used for waste management because of its beneficial qualities such as fly pest control, biomass production, and rapid organic waste degradation. Studies have uncovered the insect’s ability to tolerate MP, and consider the possibility that they may be able to degrade polymers. For the first time in Ecuador, the present study characterized <jats:italic>H. illucens</jats:italic> using the sequences of different molecular markers. Finally, <jats:italic>H. illucens</jats:italic>’ degrading capacity was evaluated in the presence of MP and decaying food residues, resembling landfill conditions.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses(Public Library of Science (PLoS), 2024-05-16) ;Diego F. Quito-Avila ;Edison Reyes-Proaño ;Gerardo Armijos-Capa ;Ricardo I. Alcalá BriseñoRobert AlvarezThis study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (<jats:italic>Bemisia tabaci</jats:italic> MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas.
