FLORES FLOR, FRANCISCO JAVIER
Preferred name
FLORES FLOR, FRANCISCO JAVIER
Main Affiliation
CIAL - Centro de Investigación de Alimentos
Web Site
ORCID
0000-0001-8092-3750
Scopus Author ID
58620465200
37 results
Now showing 1 - 10 of 37
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of Strawberry Wilt Caused by <i>Fusarium oxysporum</i> in Ecuador(Scientific Societies, 2020-05)Scopus© Citations 1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Phylodynamics of classical swine fever virus with emphasis on Ecuadorian strains(Hindawi Limited, 2018-01-10)Scopus© Citations 14 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Draft Genome Sequence of a Metronidazole-Resistant Bacteroides fragilis Strain Isolated in Ecuador(American Society for Microbiology, 2019-12-05); Marcela Bovera<jats:p> Here, we report the draft genome sequence of <jats:named-content content-type="genus-species">Bacteroides fragilis</jats:named-content> strain Z&Z143, a metronidazole-resistant bacterium isolated from a blood culture from an Ecuadorian patient hospitalized in a medical institution in Quito, Ecuador. We describe a new variant of the <jats:italic>nim</jats:italic> genes, which is associated with metronidazole resistance. </jats:p>Scopus© Citations 5 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Exploring the virome of Vasconcellea x heilbornii: the first step towards a sustainable production program for babaco in Ecuador(Springer Science and Business Media LLC, 2020-06-16); Scopus© Citations 18 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses(Public Library of Science (PLoS), 2024-05-16)Scopus© Citations 3 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Genetic diversity and organic waste degrading capacity of <i>Hermetia illucens</i> from the evergreen forest of the Equatorial Choco lowland<jats:p>Globally, microplastics (MP) represent a growing burden for ecosystems due to their increasing presence at different trophic levels. In Ecuador, the lack of waste segregation has increased the quantity of waste, primarily organics and plastics, overloading landfills and water sources. Over time, plastics reduce in size and silently enter the food chain of animals, such as insects. The black soldier fly (BSF) larvae, <jats:italic>Hermetia illucens</jats:italic> (Linnaeus, 1758), is a species with devouring behavior used for waste management because of its beneficial qualities such as fly pest control, biomass production, and rapid organic waste degradation. Studies have uncovered the insect’s ability to tolerate MP, and consider the possibility that they may be able to degrade polymers. For the first time in Ecuador, the present study characterized <jats:italic>H. illucens</jats:italic> using the sequences of different molecular markers. Finally, <jats:italic>H. illucens</jats:italic>’ degrading capacity was evaluated in the presence of MP and decaying food residues, resembling landfill conditions.</jats:p>Scopus© Citations 16 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Virosis en el cultivo del tabaco(Clinical Biotec, 2023-12-15); Felipe R. Garcés-Fiallos<jats:p>El tabaco (Nicotiana tabacum L.) es un cultivo industrial de importancia económica y una planta modelo importante, cultivada ampliamente en muchos países. Su producción, rendimiento y calidad se han visto gravemente afectados por una serie de factores entre las que destacan las infecciones virales. Hasta el momento se han reportado más de 60 virus pertenecientes a 20 géneros que infectan y provocan pérdidas sustanciales de rendimiento en tabaco. Los genomas de estos fitopatógenos pueden ser de ADN o ARN, distribuyéndose en varias partículas virales (monopartito, bipartito o tripartito). En su gran mayoría, las infecciones virales en tabaco son ocasionadas por virus de ARN monocatenarios de sentido positivo (ARN +) como los pertenecientes al género Tobamovirus y Cucumovirus. No obstante, los Begomovirus (virus de ADN) también impactan económicamente el cultivo de tabaco. Esta revisión enlista los principales virus de ADN y ARN que infectan plantas de tabaco, así como sus vectores más relevantes. Además, se abordan las técnicas de detección y diagnóstico que se han desarrollado continuamente para identificar correctamente las enfermedades virales asociadas al cultivo de tabaco. Palabras clave: Nicotiana tabacum L., virus de plantas, incidencia de virus, detección y diagnóstico viral</jats:p>Scopus© Citations 1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Near-Complete Genome Sequence of Ryegrass Mottle Virus from Irrigation Water in Ecuador(American Society for Microbiology, 2021-05-06); Kenneth M. Stedman<jats:p> In this work, we report the near-complete genome sequence of Ryegrass mottle virus identified in irrigation water through next-generation sequencing and <jats:italic>de novo</jats:italic> assembly. The genome is 4,247 bp long, arranged in five open reading frames with a 5′ untranslated region (UTR) of 87 nucleotides and a 3′ UTR of 247 nucleotides. </jats:p>Scopus© Citations 2 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Complete Genome Sequence of
<i>Alstroemeria</i>
<i>Necrotic Streak Virus</i>
Isolated from Limonium sinuatum in Ecuador(American Society for Microbiology, 2022-11-17); Simon Roux<jats:p> The complete genome sequence of <jats:italic>Alstroemeria</jats:italic> <jats:italic>necrotic streak virus</jats:italic> (ANSV) isolated from the statice cut flower ( <jats:named-content content-type="genus-species">Limonium sinuatum</jats:named-content> ) is presented. The genome has the three characteristic segments of the genus <jats:italic>Orthotospovirus</jats:italic> , family <jats:italic>Tospoviridae</jats:italic> , the S segment (3,135 bp), the M segment (4,869 bp), and the L segment (8,755 bp). </jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, First Report of Pectobacterium brasiliense Causing Banana Soft Rot in Ecuador(Scientific Societies, 2024-02-01); L. A. RamosBanana (Musa spp.) is the most economically important crop in Ecuador, with exports representing 35% of the agricultural GDP of the country. It covers 230,000 ha, mostly concentrated in three coastal provinces, Guayas, Los Ríos, and El Oro. Between July and September 2022, disease symptomatic banana cultivar Williams plants were observed in commercial plantations located in two parishes in the province of Guayas (Naranjito and Lorenzo de Garaicoa) and one parish in the province of Santo Domingo de los Tsáchilas (La Concordia), with an incidence that ranged from 5 to 15%. Symptoms included soft rot of the pseudostem and rhizome decay, characterized by a fetid odor. Three symptomatic pseudostems from each location were collected, washed with running water to remove any debris, and dried with absorbent paper. From the lesion of each pseudostem, seven pieces of 2 cm2 were taken, surface sterilized, and macerated in 9 ml of sterile peptone water (0.1% w/v). The macerate was diluted threefold in sterile water, plated on nutrient agar, and incubated at 30°C for 24 h. Eight randomly picked colonies, with convex elevation and creamy white color, were isolated on nutrient agar. Each of the bacterial isolates was biochemically profiled by the Biolog system (Biolog, U.S.A.) and identified as Pectobacterium. Three isolates, one from each parish (FP220416, FP220694, and FP220904), were selected for testing Koch’s postulates and further identification. Sequences from fragments of the 16S, dnaA, gapA, and gyrB genes were obtained from these isolates, following the protocols used by Dobhal et al. (2020) and Boluk et al. (2020), showing 98.1 to 99.0%, 98.2%, 99.7 to 99.8%, and 98.4 to 98.9% identity, respectively, with sequences from the Pectobacterium brasiliense type strain LMG_21371 (accession no. JQOE00000000). The obtained sequences were deposited in GenBank with the following accession numbers: OR392417, OR371545, OR371546, OR727281, OR727282, and OR739074 to OR739080. Using BEAST version 1.10.4 (Suchard et al. 2018), a Bayesian multilocus phylogenetic tree was built with multiple sequence alignments of dnaA, gapA, and gyrB from 22 P. brasiliense isolates and two P. aquaticum isolates used as outgroup. The phylogenetic analysis showed that the Ecuadorian isolates cluster with P. brasiliense BF20 isolated from Opuntia ficus-indica in Mexico and are closely related with the type strain. Pathogenicity tests were conducted through syringe infiltration with 1 ml of 1 × 108 CFU/ml bacterial suspension. Each of the three characterized isolates were inoculated into the pseudostems of five healthy 4-month-old banana plants of the cultivar Williams. Negative control plants were infiltrated with sterile distilled water. The plants were incubated at 25°C and 74% relative humidity. Black lesions started to appear 11 days after inoculation, and 5 weeks after inoculation plants showed clear symptoms of soft rot of the pseudostem, including fetid odor associated with plant tissue decomposition. The control plants remained symptom-free. Bacteria were reisolated only from symptomatic pseudostems and identified as P. brasiliense with the specific primers Pb1F and Pb1R.Scopus© Citations 1