The E1a Adenoviral Gene Upregulates the Yamanaka Factors to Induce Partial Cellular Reprogramming

Gracia Mendoza; GONZALEZ PASTOR, REBECA EUGENIA; Juan Miguel Sánchez; Altamira Arce-Cerezo; Miguel Quintanilla; Gema Moreno-Bueno; Anna Pujol; Carolina Belmar-López; Alba de Martino; Efrén Riu; Tristan A. Rodriguez; Pilar Martin-Duque

doi:10.3390/cells12091338

The E1a Adenoviral Gene Upregulates the Yamanaka Factors to Induce Partial Cellular Reprogramming

Journal

Cells

ISSN

2073-4409

Date Issued

2023-05-07

Author(s)

Gracia Mendoza

GONZALEZ PASTOR, REBECA EUGENIA

Facultad de Ciencias de la Salud Eugenio Espejo

Juan Miguel Sánchez

Altamira Arce-Cerezo

Miguel Quintanilla

Gema Moreno-Bueno

Anna Pujol

Carolina Belmar-López

Alba de Martino

Efrén Riu

Tristan A. Rodriguez

Pilar Martin-Duque

DOI

10.3390/cells12091338

URL

https://cris.ute.edu.ec/handle/123456789/357

Abstract

The induction of pluripotency by enforced expression of different sets of genes in somatic cells has been achieved with reprogramming technologies first described by Yamanaka’s group. Methodologies for generating induced pluripotent stem cells are as varied as the combinations of genes used. It has previously been reported that the adenoviral E1a gene can induce the expression of two of the Yamanaka factors (c-Myc and Oct-4) and epigenetic changes. Here, we demonstrate that the E1a-12S over-expression is sufficient to induce pluripotent-like characteristics closely to epiblast stem cells in mouse embryonic fibroblasts through the activation of the pluripotency gene regulatory network. These findings provide not only empirical evidence that the expression of one single factor is sufficient for partial reprogramming but also a potential mechanistic explanation for how viral infection could lead to neoplasia if they are surrounded by the appropriate environment or the right medium, as happens with the tumorogenic niche.