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Author: search.filters.author.R. BorjaHas files: Yes

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    Valorization of Lignocellulosic Biomass Through Anaerobic Digestion after the Cultivation of the Edible Mushroom Lentinula Edodes and Enzymatic Pretreatment
    (Springer Science and Business Media LLC, 2025-08-07)
    O. H. López Balladares
    ;
    D. De la Lama Calvente
    ;
    F. J. Flores-Flor
    ;
    R. Borja
    The global scarcity of high-quality protein and food and the imminent effects of climate change have pushed forward scientific efforts to provide sustainable and efficient responses. The use of by-products at industrial facilities has been presented as the most promising alternative to mitigate these global problems. Agricultural byproducts as substrates for the growth of edible food and the subsequent valorization of the non-edible leftovers through anaerobic digestion fulfil this perspective. The aim of this study was, for the first time, to assess the biomethanation potential of four lignocellulosic by-products (sugarcane bagasse, cocoa husks, coconut shells, and rice hulls) used as substrates mixed with an anaerobic digestate for the cultivation of Lentinula edodes. In addition, enzymatic pretreatment by Trichoderma spp. was also investigated. The highest methane yield (129 ± 9 NLCH4 kg− 1VS) and the highest maximum methane production rate (9.8 ± 0.7 NLCH4 kg− 1VS d− 1) were observed for the substrate moistened with the anaerobic digestate and the subsequent enzymatic pretreatment. However, the highest kinetic constants of the first-order model were achieved without enzymatic pretreatment (0.090 and 0.091 d− 1). This study highlights the viability of a closed-loop process where otherwise wasted biomass is used to produce edible mushrooms and biogas, a process embedded in a circular economy model.
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    Batch mesophilic anaerobic digestion of mixtures of lignocellulosic biomasses from Ecuador: influence of inoculum type and thermal pretreatment on methane production and process kinetics
    (Springer Science and Business Media LLC, 2025-04-21)
    O. H. López-Balladares
    ;
    D. De la Lama-Calvente
    ;
    FLORES FLOR, FRANCISCO JAVIER  
    ;
    R. Borja
    Comprehensive and integral treatment of agricultural wastes is crucial to palliate the environmental impact associated with uncontrolled disposal. However, waste management should be focused on a local perspective. In this study, the anaerobic co-digestion of four lignocellulosic by-products generated in Ecuador (cocoa husks, coconut shells, rice hulls, and sugarcane bagasse) was evaluated. Two different mixture ratios, the impact of thermal pretreatment, and the influence of the inoculum origin were also assessed. Results showed that the anaerobic inoculum collected from a brewery factory showed the highest efficiency. Moreover, thermal pretreatment improved the methane yields (158–160 NLCH4 kg−1VS). However, the highest kinetic constant (1.1 ± 0.1 d−1; first-order kinetic model) and the maximum methane production rate (153 ± 3 NLCH4 kg−1VS d−1; logistic model) were obtained for the untreated mixtures. Finally, the substrate mixture ratio did not influence the anaerobic digestion performance. Therefore, the present study provides preliminary insights into the viability of anaerobic co-digestion as a feasible technology for the valorization of agro-industrial lignocellulosic residues to reduce the effect of seasonal production variations.