MIDEROS MORA, MARÍA CRISTINA
Preferred name
MIDEROS MORA, MARÍA CRISTINA
Main Affiliation
SF
Scopus Author ID
57211652834
3 results
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Item type:Publication, Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases(Springer US, 2019-11-10) ;Laura Miguel-Romero; ;Alberto MarinaPatricia Casino - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Author Correction: Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases(Springer Science and Business Media LLC, 2020-05-19); ;Laura Miguel-Romero ;Alonso Felipe-Ruiz ;Patricia CasinoAlberto Marina<jats:p>An amendment to this paper has been published and can be accessed via a link at the top of the paper.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases(Springer Science and Business Media LLC, 2020-02-07); ;Laura Miguel-Romero ;Alonso Felipe-Ruiz ;Patricia CasinoAlberto Marina<jats:title>Abstract</jats:title><jats:p>Histidine is a versatile residue playing key roles in enzyme catalysis thanks to the chemistry of its imidazole group that can serve as nucleophile, general acid or base depending on its protonation state. In bacteria, signal transduction relies on two-component systems (TCS) which comprise a sensor histidine kinase (HK) containing a phosphorylatable catalytic His with phosphotransfer and phosphatase activities over an effector response regulator. Recently, a pH-gated model has been postulated to regulate the phosphatase activity of HisKA HKs based on the pH-dependent rotamer switch of the phosphorylatable His. Here, we have revisited this model from a structural and functional perspective on HK853–RR468 and EnvZ–OmpR TCS, the prototypical HisKA HKs. We have found that the rotamer of His is not influenced by the environmental pH, ruling out a pH-gated model and confirming that the chemistry of the His is responsible for the decrease in the phosphatase activity at acidic pH.</jats:p>
