CRIS

Permanent URI for this communityhttps://cris.ute.edu.ec/handle/123456789/1

Browse

Settings

Subject: search.filters.subject.CRISPR/Cas9

Search Results

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Microdroplet Systems for Gene Transfer: From Fundamentals to Future Perspectives
    (MDPI AG, 2025-10-31)
    Criollo, Mishell
    ;
    Gina Layedra
    ;
    Camilo Pérez Sosa
    ;
    Gustavo Rosero
    ;
    PEÑAHERRERA PAZMIÑO, ANA BELÉN  
    Microfluidics enables precise control of fluid movement within microchannels, facilitating the generation of microdroplets at high frequencies. This technology provides a unique platform for conducting biological and chemical experiments, enhancing throughput and sensitivity, particularly in single-cell analysis. The microdroplet environment enhances interactions between cells and gene delivery materials, resulting in greater contact area, higher reagent concentration, and improved diffusion for both eukaryotic and prokaryotic cells. This review discusses the advantages and limitations of transfection and transformation within microdroplet technologies, highlighting their potential to improve gene editing efficiency while addressing challenges related to delivery mechanisms and cellular uptake rates. The integration of microdroplet technology with advanced gene editing tools, such as CRISPR/Cas9, promises to streamline processes and improve outcomes in various applications, including therapeutic interventions, vaccine development, regenerative medicine, and personalized medicine. These advancements could lead to more precise targeting of genetic modifications, resulting in tailored therapies that better meet individual patient needs. Overall, the integration of gene delivery in microdroplets represents a significant leap in biotechnology, enhancing the efficacy of gene delivery systems and opening new avenues for research and development in precision medicine.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Advances in environmental biotechnology with CRISPR/Cas9: bibliometric review and cutting-edge applications
    (Springer Science and Business Media LLC, 2025-02-22)
    Jessica Verdezoto Prado
    ;
    Cristhian Chicaiza Ortiz
    ;
    Ana Belén Mejía Pérez
    ;
    Camila Freire Torres
    ;
    Marco Viteri Yánez
    CRISPR/Cas9 has emerged as the predominant method for genome editing due to its cost-effectiveness and broad applicability, playing a crucial role in advancing sustainable practices across various sectors. This systematic review employs the PRISMA methodology to evaluate the impact of CRISPR/Cas9 on environmental protection and on achieving Sustainable Development Goals (SDGs) such as SDG 2, 3, 6, 7, 9, 12, 13, and SDG15. These goals focus on the responsible use of natural resources, reducing the negative effects of climate change and ensuring safe food for the entire population. Analyzing data from the Web of Science, the review found significant growth in related publications, with a 30% increase since 2014, predominantly from the US, China, Germany, and the UK. The study categorizes the scientific developments into these trends, the enhancement of plant tolerance to environmental stresses, as evidenced by the consistent focus on terms such as “tolerance” and “plant” since 2021. Furthermore, the relevance of “Gene Editing” has increased significantly since 2022, underscoring the importance of CRISPR/Cas9 in developing resilient crops that can withstand extreme conditions. These trends underscore the growing significance of biotechnological advancements in the mitigation of climate change’s effects and the improvement of ecosystem stability. Key discussions include CRISPR/Cas9’s role in the development of fourth-generation biofuels and environmental biosensors, as well as its applications in enhancing genetic resilience and controlling invasive species. These innovations highlight CRISPR/Cas9’s potential in promoting sustainable resource management and energy generation, making a significant contribution to ecological conservation and sustainability efforts.