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Author: search.filters.author.Saeed ShokriLanguage: search.filters.language.English

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    Resveratrol protects against isoflurane-induced testicular injury: a stereological and molecular study in mice
    (Elsevier BV, 2026-01)
    Zahra Mohammadi
    ;
    Sanaz Alaee
    ;
    Majid Kamali-Dolat Abadi
    ;
    Somayyeh Karami-Mohajeri
    ;
    Zahra Khodabandeh
    Background This study examines the therapeutic effects of resveratrol, known for its antioxidant and anti-inflammatory properties, in mitigating the harmful effects of isoflurane, an inhalation anesthetic, on testicular tissues in mice. Methods Sixty adult male C57BL/6 mice were grouped into six categories: control (normal saline), isoflurane only (1.5% for 1-hour/day), low-dose Resveratrol (RSV, 50 mg/kg/day), high-dose RSV (100 mg/kg/day), isoflurane combined with low-dose RSV, and isoflurane combined with high-dose RSV. After 35-days, the mice were euthanized, and their testes were harvested for stereological analysis and the assessment of antioxidant and apoptotic markers. Results Exposure to isoflurane resulted in decreased seminiferous tubule volume and length, along with increased interstitial tissue volume of the testis. Co-treatment with resveratrol, especially at higher doses, mitigated these changes. Additionally, resveratrol modulated the expression of the apoptotic genes Bcl2l1, Bax , and Caspase-3 , and enhanced the expression of antioxidant genes GPX1, Sod1 , and catalase in mice that received isoflurane. Conclusions The findings suggest that resveratrol effectively counters isoflurane-induced damage in testicular structures and gene expression, enhancing tubular integrity and antioxidant defense. This indicates a potential therapeutic approach to protect against isoflurane-induced testicular toxicity.
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    Gallic acid mitigates cadmium-induced reproductive damage: Effects on sperm, antioxidants, and apoptosis in mice
    (Elsevier BV, 2025-12)
    Sanaz Alaee
    ;
    Zahra Derakhshan
    ;
    Farhad Koohpeyma
    ;
    Saeed Shokri
    ;
    Amirreza Talaie
    Cadmium is a well-known reproductive toxicant found in many industrial wastes. The gallic acid impact on the recovery of reproductive ability of mice exposed to cadmium was investigated. The experiment involved six groups of male mice, designated as Groups 1 through 6, each receiving specific treatments: Group 1 received distilled water, Group 2 received water containing gallic acid with a concentration of 50 mg/kg, Group 3 received water containing gallic acid with a concentration 100 mg/kg, Group 4 was treated with water containing cadmium with a concentration of 1.2 mg/kg, Group 5 received water containing cadmium and gallic acid with concentrations of 1.2 mg/kg and 50 mg/kg, respectively, and finally Group 6 received water containing 1.2 and 100 mg/kg of cadmium and gallic acid, respectively. The mice's blood samples were collected after finishing the treatment stage to evaluate testosterone levels, and the testes were excised for stereological analysis and assessment of antioxidant and apoptotic marker expression. Sperm samples extracted from the epididymis were analyzed for quantity, morphology, motility, viability, maturation, and DNA fragmentation. Results indicated significant declines in all measured parameters in mice exposed to cadmium. Additionally, high doses of gallic acid adversely affected some fertility parameters in normal mice. In cadmium-exposed mice, mRNA expression of antioxidants (Gpx1, Cat, and Sod1) and anti-apoptotic (Bcl2l1) genes was significantly reduced, while the expression of proapoptotic (Caspase3 and Bax) genes increased. Notably, 50 mg/kg of gallic acid improved all fertility parameters in these mice. It is suggested that the high concentration of antioxidants can control oxidative stress, thereby disrupting the normal physiological level of reactive oxygen species in the mice's cells. Therefore, gallic acid at the appropriate dose improves fertility parameters in mice exposed to cadmium. The results showed that the dose of 50 mg/kg of gallic acid can effectively mitigate the cadmium detrimental impacts on the health of the vital organs of the male mice reproductive system. These findings suggest the potential therapeutic application of gallic acid in combating reproductive toxicity induced by cadmium, highlighting the importance of dosage in achieving beneficial outcomes.