Resveratrol protects against isoflurane-induced testicular injury: a stereological and molecular study in mice
Journal
Clinics
ISSN
1807-5932
Date Issued
2026-01
Author(s)
Zahra Mohammadi
Sanaz Alaee
Majid Kamali-Dolat Abadi
Somayyeh Karami-Mohajeri
Zahra Khodabandeh
Saeed Shokri
Sulagna Dutta
Pallav Sengupta
Abstract
Background
This study examines the therapeutic effects of resveratrol, known for its antioxidant and anti-inflammatory properties, in mitigating the harmful effects of isoflurane, an inhalation anesthetic, on testicular tissues in mice.
Methods
Sixty adult male C57BL/6 mice were grouped into six categories: control (normal saline), isoflurane only (1.5% for 1-hour/day), low-dose Resveratrol (RSV, 50 mg/kg/day), high-dose RSV (100 mg/kg/day), isoflurane combined with low-dose RSV, and isoflurane combined with high-dose RSV. After 35-days, the mice were euthanized, and their testes were harvested for stereological analysis and the assessment of antioxidant and apoptotic markers.
Results
Exposure to isoflurane resulted in decreased seminiferous tubule volume and length, along with increased interstitial tissue volume of the testis. Co-treatment with resveratrol, especially at higher doses, mitigated these changes.
Additionally, resveratrol modulated the expression of the apoptotic genes Bcl2l1, Bax , and Caspase-3 , and enhanced the expression of antioxidant genes GPX1, Sod1 , and catalase in mice that received isoflurane.
Conclusions
The findings suggest that resveratrol effectively counters isoflurane-induced damage in testicular structures and gene expression, enhancing tubular integrity and antioxidant defense. This indicates a potential therapeutic approach to protect against isoflurane-induced testicular toxicity.
This study examines the therapeutic effects of resveratrol, known for its antioxidant and anti-inflammatory properties, in mitigating the harmful effects of isoflurane, an inhalation anesthetic, on testicular tissues in mice.
Methods
Sixty adult male C57BL/6 mice were grouped into six categories: control (normal saline), isoflurane only (1.5% for 1-hour/day), low-dose Resveratrol (RSV, 50 mg/kg/day), high-dose RSV (100 mg/kg/day), isoflurane combined with low-dose RSV, and isoflurane combined with high-dose RSV. After 35-days, the mice were euthanized, and their testes were harvested for stereological analysis and the assessment of antioxidant and apoptotic markers.
Results
Exposure to isoflurane resulted in decreased seminiferous tubule volume and length, along with increased interstitial tissue volume of the testis. Co-treatment with resveratrol, especially at higher doses, mitigated these changes.
Additionally, resveratrol modulated the expression of the apoptotic genes Bcl2l1, Bax , and Caspase-3 , and enhanced the expression of antioxidant genes GPX1, Sod1 , and catalase in mice that received isoflurane.
Conclusions
The findings suggest that resveratrol effectively counters isoflurane-induced damage in testicular structures and gene expression, enhancing tubular integrity and antioxidant defense. This indicates a potential therapeutic approach to protect against isoflurane-induced testicular toxicity.