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Author: search.filters.author.Manikantan PappuswamySubject: search.filters.subject.Bioremediation

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    Item type:Publication,
    Appraisal of the potential of endophytic bacterium Bacillus amyloliquefaciens from Alternanthera philoxeroides: A triple approach to heavy metal bioremediation, diesel biodegradation, and biosurfactant production
    (Elsevier BV, 2024-10)
    Soma Biswas
    ;
    Saranya Jayaram
    ;
    Indhu Philip
    ;
    Balamuralikrishnan Balasubramanian
    ;
    Manikantan Pappuswamy
    Endophytic microbes have been associated with many positive traits due to their endurance mechanisms. The current study was designed at exploring the potential of the endophytic bacterium Bacillus amyloliquefaciens MEBAphL4 isolated from Alternanthera philoxeroides for biosurfactant production and bioremediation efficiency. This endophyte, isolated from the polluted Madiwala lake in Bangalore, displayed elevated resistance to Cr and Pb till 2000 mg/L. The metal removal efficiency was found to be higher for Cr (25.7 %) at pH 6 and for Pb (92.3 %) at pH 9. Further, the present study also describes biosurfactant production with good emulsification ability (E24-52 %) and stability over a range of pH (8−12), temperature (20–40°C) and salinity (5–15 %). Biosurfactant production was enhanced 1.18-fold using the Response Surface Methodology approach and characterised by Fourier Transformation Infra-red Spectroscopy and Ultra-Performance Liquid Chromatography- Mass Spectrometry showing the presence of lipopeptides, fengycin, iturin and surfactin of molecular weights 1463.65, 1043.44 and 1012.56 Da respectively. The potential application of the biosurfactant in degrading various hydrocarbons was evaluated, demonstrating its effectiveness in bioremediation of oil-contaminated sites. Specifically, diesel biodegradation was measured at 56.46±0.95 %. These findings underscore the potential of B. amyloliquefaciens in environmental applications such as heavy metal biosorption and the bioremediation of contaminated sites, particularly those affected by oil spills and correlates to UN SDG6 of clean water and sanitation.