ZUÑIGA MIRANDA, JOHANA JANINAJOHANA JANINAZUÑIGA MIRANDAGONZALEZ PASTOR, REBECA EUGENIAREBECA EUGENIAGONZALEZ PASTORSaskya E. Carrera PachecoCristina Rodríguez-PólitCarlos Barba-OstriaAntónio MachadoGUAMAN BAUTISTA, LINDA PRISCILALINDA PRISCILAGUAMAN BAUTISTAChristian D. Alcivar-LeónHEREDIA MOYA, JORGE HUMBERTOJORGE HUMBERTOHEREDIA MOYA2025-07-212025-07-212025-01-31https://doi.org/10.1007/s42452-025-06459-7Schiff bases are organic compounds recognized for their biological activities, including antiviral, antibacterial, antifungal, and anticancer properties, making them promising candidates in medicinal chemistry. In this studio, a series of Schiff bases derived from 4-aminoantipyrine and substituted cinnamaldehydes were evaluated in vitro against liver (HepG2) and thyroid (THJ29T) cancer cells, Gram-positive and Gram-negative multidrug-resistant bacteria, and biofilm-forming pathogens. Six compounds demonstrated anticancer activity, though some exhibited toxicity to non-tumor cells. Compounds showed notable anticancer potential, while also exhibited strong antibacterial effects, with being the most effective against multidrug-resistant bacteria strains. These Schiff bases also inhibit biofilm formation, suggesting their potential for treating biofilm-related infections. analyses of their ADME properties, global reactivity descriptors, and binding affinities corroborated these findings. The Schiff base has a strong binding affinity for DNA gyrase and vitamin D receptor, suggesting potential mechanisms for its antibacterial and anticancer activities.en4-aminopantipyrineAntibacterialAnticancerMultidrug-resistantSchiff basetext::journal::journal article